CROP-Seq: Single-cell CRISPR screening

Learn how the CROP-Seq technology at Myllia can help to elucidate novel drug targets at single-cell resolution

CROP-seq (or CRISPR Droplet Sequencing) enables high-throughput functional genomics by combining CRISPR gene editing and single-cell RNA sequencing. CROP-seq uses a pool of guide RNAs that target different genes of interest. These guide RNAs are introduced into individual cells using lentiviral transduction, allowing for the parallel introduction of genetic perturbations into thousands of cells.

Finally, single-cell RNA-sequencing and the bioinformatic analysis of NGS data allows to study phenotypic effects of those genetic modifications.