Design of highly efficient sgRNA libraries for CRISPRn screens
The design of sgRNAs for CRISPRn, CRISPRi and CRISPRa is a crucial factor determining the success of CRISPR screening experiments. Myllia´s proprietary sgRNA design algorithm selects highly active sgRNA sequences for targeting both essential and non-essential genes and appears to be “superior” to other publicly available libraries.
Myllia´s sgRNA design algorithm selects highly active sgRNA sequences for targeting both essential and non-essential genes and appears to be “superior” to other publicly available libraries.
Essentially, we have performed CRISPR screens to target essential and non-essential genes using two different read-outs to validate the high activity Myllia’s sgRNA designs:
– Dropout screen based on essentiality targeting 24 genes
– FACS-based screen with a CD59 receptor read-out targeting 9 genes
We will expand the screening campaign to target additional non-essential genes that may help further improve sgRNA design and increase editing rates. Download the full CRISPRn sgRNA design slide deck highlighting all relevant results for the dropout and FACS-based screens: