Poster | A compressed genome-wide CROP-Seq screen to dissect the polarization of THP-1-derived macrophages
Here, we present a genome-wide CROP-Seq screen to identify genes involved in macrophage polarization. We used a compressed CROP-Seq paradigm in which THP-1 monocytes were loaded with multiple sgRNAs.
CRISPR screens are a powerful discovery engine for biological research and are used for drug target discovery and target validation. Pooled screens are advantageous because they can be performed at genome-scale. Especially, Perturb-Seq/CROP-Seq screens are an attractive approach as they combine pooled CRISPR perturbations with single-cell RNA sequencing (scRNA-Seq) as a high-content phenotyping readout.
Here, we present a genome-wide CROP-Seq screen to identify genes involved in macrophage polarization. We used a compressed CROP-Seq paradigm in which THP-1 monocytes were loaded with multiple sgRNAs, differentiated towards M0 macrophages and polarized into the M1 subtype. M1 macrophage polarization was assessed by scRNA-Seq, focusing on a pre-defined signature of 395 mRNAs.